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This approach was successfully applied to the sequencing of both discontinuous exonic sequences, and contiguous genomic regions [21], [22], [23].
Sequencing reactions were repeated with alternate primers as needed to fill any gaps present to complete the sequencing of both DNA strands in each amplicon.
This indicates that to approach full transcriptome information, the sequencing of both types of libraries is necessary.
MethylC-seq and RRBS require the sequencing of both the untreated and the bisulfite treated genomes in order to compare them and identify methylated cytosines.
The sequencing of both sexes (e.g., non-recombining and sex-determining regions) provides important markers and crucial data for evolutionary and biological inferences.
The efficacy of combined radiotherapy and adenoviral therapy does not seem to be dependent on the sequencing of both agents, but needs to be further explored within the same tumour model.
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Relying on the known structures of GroEL and GroES, sites for attachment of fluorescent probes are designed into the sequence of both proteins.
Figure 2 A frequency distribution for different size classes of recipient genome deletions among 13 T-DNA integration sites for which the sequences of both junctions were determined.
"Now that we've got the sequence of both sex chromosomes, we can do a very detailed comparison" of male and female, says Ross.
We show that the problem is strongly NP-hard, and develop an O(n3) polynomial time algorithm to solve the special case where the sequences of both types of jobs are given.
The sequences of both Annexin A2-WT and Annexin A2-K302A were cloned into pcs4 (C-myc-tag at C-terminal), with resulting vectors called pcs4-Annexin A2-WT-myc and pcs4-Annexin A2-K302A-myc A2-K302A-myc A2-K302A-myc
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com