DNA sequencing was carried out on PCR products with the PRISM Sequencing Kit and the 377A sequencing apparatus from Applied Biosystem (Warrington) according to the manufacturer recommendation.
The primers and PCR conditions are detailed in table 1. Product sizes were evaluated by agarose gel electrophoresis and amplicons were sequenced in both directions using the Big Dye terminator sequencing kit (Applied Biosystems) on ABI PRISM sequencing apparatus 3730 (Applied Biosystems).
The sequence reactions were delineated at Uppsala Genome Center on an ABI PRISM 3730xl sequencing apparatus (Applied Biosystems).
Dye terminators were removed using the CleanSEQ kit (Agencourt Biosciences), and sequence reactions were run on ABI PRISM 3730xl sequencing apparatus (Applied Biosystems, Foster City, CA, USA).
Stephen Turner (Pacific Biosciences, Foster City, USA) described the striking improvements in sequencing that they have achieved using nanopore or single-molecule sequencing apparatus.
For goods like a sequencing apparatus, this research can be even more difficult.
If you're selling a new form of sequencing apparatus, you should determine not only how many scientists worldwide are using this type of apparatus, but also how many will be buying new sequencers in the next few years.
PCR amplification and PIK3CA sequencing were performed using a 384-capillary automated sequencing apparatus (Spectrumedix, State College, PA, USA).
AJ005592-AJ005597) and their flanking intron sequences were amplified by PCR and directly sequenced using an automated sequencing apparatus (3100 Genetic Analyzer, Applied Biosystems, Foster City, CA).
