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The sequences of each gene or region were aligned by MUSCLE with default parameters.
Raw image files were processed by Illumina pipeline for base-calling with default parameters and the sequences of each individual were generated as 100-bp paired-end reads.
The sequences of each individuals were then mapped into the pseudomolecules of both parents, with the short reads mapping tools, including Maq (Li et al. 2008), SOAP2 (Li et al. 2009), SSAHA2, and SMALT (Ning et al. 2001).
Fig. 7 OOGP decommissioning options and methods simulation demonstration In addition to visualizing the sequences of each methodology, the exact time for the different phases, for example, at what time the living quarter was under lifting can also be visualized.
The sequences of each group were aligned to obtain sequence logo of the translation initiation site using WebLogo 3, version 2.8.2 (http://weblogo.berkeley.edu/).edu/
To evaluate the result, we constructed the phylogeny of nine H. pylori strains based on the sequences of each of 40 core genes of H. pylori (Table S1).
The sequences of each pair of 5' miR-RACE and 3' miR-RACE PCR products of every one of the nine ptr-miRNAs were spliced to generate the whole mature miRNA sequences (Table 3).
Where two var2csa-type genes exist in one genome, the sequences of each gene do not seem to be more closely related to each other than either is to any other randomly compared var2csa-type sequence in a different genome.
In this, the sequences of each and every amino acid position was assigned either helix or coil or extended sheets only when at least 50% of the above web servers predicted the similar secondary structure.
The sequences of each family were extracted with VMATCHSELECT.
The sequences of each primer are shown in Table 2.
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