The sequence of primers, expected product size and references are given in Table 3.
In semesters when time allowed, the PCR product was cloned (TOPO PCR Cloning, Life Technologies, Grand Island, NY) and sequenced (ACGT, Wheeling, IL) to validate the sequence of the expected PCR product and revealed that PCR using the designed primers amplified the predicted product sequence.
The sequence of the primers, expected amplicons size and annealing temperatures are summarized in Table S1.
These results were detailed into protocols and documentation, including the sequence of events and expected progress of the patients over time.
However, the scattering curve changes upon complex formation of UspA1 527 665) with N-CEACAM1, and is consistent with a mono-disperse solution with an estimated molecular mass that agrees well with the value predicted from the primary sequence of the expected complex.
The surrounding sequences show the sequence similarity expected of intergenic regions of the two species 80-900%).
Table 1 The sequence of primers and the expected size of the amplified products.
Moreover an histidine residue, located in the immediate vicinity of Trp in the sequence of both peptides, is expected to be charged at pH 5.0.
Who could have expected the sequence of adventures, conspiracies and improbable coincidences that would keep them apart for 20 years?
