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To further understand the role that the sequence in front of the armadillo repeats within APC222 653 plays in Cdc42 interaction, we made two shorter constructs: one without the armadillo repeats (APC222 453) and the other with only the armadillo repeats of APC222 653 (APC453–653), both tagged with mRFP.
The sequence in front of the loxP or lox511 arrowhead as shown in Figures 2 and 3 is retained after the recombination event that creates the deletion (see also reference [ 9]).
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The Epsom winner is generally the 11-10 favourito to continue the Ballydoyle sequence, in front of Libertarian at around 7-2, wiTradinging Leather, Sugar Boy and Galileo Rock vying for third spot.
Researchers in a few years will have trouble imagining how we studied human biology without the genome sequence in front of us.
The authors cloned the Trib1 coding sequence in front of the thyroxin-binding globulin (TBG) liver-specific promoter and established stable liver-specific overexpression using AAV serotype 8, known for its high liver specificity and affinity [ 24].
The P-plasmid was constructed by cloning the 2 kb sequence in front of the first exon of Olig2 (mm8_chr16:91,112,391 — 91,114,400) upstream of the GFP gene on the pEGFP plasmid.
A BamHI site is present 3' of the E-tag sequence in front of the stop codon when no tag is to be attached.
With the aim of changing the sequence precisely in front of the translation initiation codon ATG, in accordance to Kozak [ 33] and Luetcke et al. [ 34], the five nucleotides CTCAA were inserted between the restriction sites XhoI and NcoI (NcoI including the translation initiation codon) of pluc to give pluc-enh (Fig. 1A).
Indeed, the canonical Shine Dalgarno sequence in front of cyanobacterial genes is rarely found in the typical position in front of chloroplast open reading frames, if it is found at all [reviewed in [ 76, 77]].
It had this thundery Bernard Herrmann score, and when Robert De Niro did his "are you talking to me?" sequence in front of the mirror you suddenly sensed the degree of anger there.
KM performed the evaluation of lysis systems, KA constructed the vectors used in this work, MNakamura evaluated the effect of introducing SD-like sequence in front of ATG for green-light induction.
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CEO of Professional Science Editing for Scientists @ prosciediting.com