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Eight pairs of primers based on the sequence characterized amplified region (SCAR) were designed and successfully converted to SCAR markers, which were used to sex progeny from different crosses of known genotypes for validation.
Primers Fg16F (5′-CTCCG-GATATGTTGCGTCAA-3′) and Fg16R (5′-GGTAGGTATCCGACATGGCAA-3′) were used as a pair of specific PCR primers to determine if a Fusarium isolate belongs to a FGSC isolate, and sizes of this PCR amplicons were used to assign the sequence characterized amplified region (SCAR) types to each isolate (Carter et al. 2000; Nicholson et al. 1998; Carter et al. 2002).
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The ctg1034 sequence characterized in the present study is a good example of such high conservation of structure.
Of the 77 sequence characterized loci, 27 had only one EST unigene while the remaining 50 had more than one EST unigene.
In order to increase the number of sequence characterized markers on the map and diversify the type of markers, AFLP was used [ 32].
Interestingly, the CcCP4 cDNA sequence characterized contains the C-terminal sequence KDDL.
Standard AFLP using MseI with the methylation sensitive PstI was used to further enrich the map with sequence characterized markers.
For the development of sequence characterized amplified region (SCAR) markers, a 747 bp amplicon was generated by the ISSR method and sequenced.
The new DNA sequence characterized on chromosome IIα was also found to be present at chromosome IVβ extremity.
The first repetitive DNA sequence characterized in Leishmania corresponded to the telomeric repeats [ 13].
Physical mapping started with screening the hermaphrodite BAC library using the sex co-segregating sequence characterized amplified region (SCAR) marker W11 by which four positive BACs were identified.
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