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But in many cases, it is not clear how the sequence changes alter gene function — if at all.
Some property differences of the isomers caused by the sequence changes were found.
To effect a desired genomic change the sequence changes of interest are incorporated between two adjacent borders homologous to F. graminearum DNA which will target them to the desired location.
Dark blue arrows indicate the sequence changes in one of the TRIM lines.
Some of the sequence changes were then validated by the dye-terminator method.
The resulting fragments differ in size and mass depending on the sequence changes generated through bisulfite treatment.
Possible reasons could be that there are different selective pressures influencing the timing of when the sequence changes occur in the two groups.
The sequence changes recruiting or modifying regulatory elements did not necessarily extinguish the original expression patterns of their associated genes in other tissues.
With high-throughput sequencing, several studies investigated the sequence changes in rice Tos17 mutant lines or Arabidopsis regenerated plants by NGS strategies.
Nevertheless, according to the above-mentioned work, the sequence changes considerably in single-element system that was not the case for the present study.
Each of the sequence changes observed in these 10 errant HVI∞M2 samples were non-heteroplasmic at each altered position.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com