Exact(1)
The population of parental tester cells in the lawn of the selection plated was determined by taking three agar plugs from the surface of the selection plates, avoiding any Lac+ colonies.
Similar(58)
Sodium chloride content in the selection plate was affected to FR901379 production and shrinkage of the colony size was observed in high producing strains.
Longer incubation of the selection plates often produces a large increase in the number of colonies.
In each set of experiments, absence of growth of the parent and the recipient strains in the selection plates was confirmed.
Moreover, the 40 colonies examined via sequencing, were a small fraction of the clones surviving the selection, with approximately 1000 to a lawn of colonies remaining on the selection plates.
However, water, wildlife feces, soil and other environmental samples often yielded growth of many non-target bacterial CFU on the selection plates that interfered with identification and selection of authentic EcO157 CFU. Background flora can decrease the sensitivity of any isolation method, thus resulting in an apparent incidence of EcO157 lower than may exist.
The selection plates were incubated at 37 °C for 24 h.
Colonies from yeast transformed with pACTDdRACK1 and pAS vector did not grow on the selection plates.
Observing a clear Luria Delbruck distribution would require preventing tester growth on the selection plate.
At 4 days after transformation of the respective plasmids by ATMT, we observed colonies on the selection plates.
After 4 days of incubation, eight fungal colonies per 10 conidia were obtained from the selection plates.
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