After incubation, the solution on the serosal side of the segment was collected.
The group of the first segments were collected in the Subset-1.
The intestine segments were collected and frozen at −80°C.
Sediment cores (subdivided into 5 cm segments) were collected in the aquifer and the sediment and porewater were used for microcosm experiments (50 experiments) and for determination of solid organic carbon, solid water partitioning coefficients, specific phenoxy acid degraders and porewater chemistry.
To investigate the effect of IO on epithelial barrier function, gut segments were collected for the measurement of transmural electrical conductance and HRP flux.
Histology and immunohistochemistry. Ileum segments were collected at the same time as plasma and other tissues, and were fixed in PBS/4% paraformaldehyde and embedded in paraffin blocks.
For each soybean line, approximately 30 seedlings were inoculated on the roots with P. sojae and after 5 days, 7.5 mm root segments were collected from immediately above and below the upper margin of the visible lesion (referred to from here as the "Upper" and "Lower" infection courts).
'Floating' 40- μm thick cryosections of thoracic and lumbar segments were collected for all of the nine cases and placed in phosphate buffered saline (PBS) in a 24-well plate.
Caecum, proximal P1, P2, middle and distal colon segments were collected precisely from standardised colon regions; caecum (the tip of caecum), P1 (1 cm from the caecum-colon junction), P2 (the end of the V-shaped mucosal folds), middle colon (4 cm from the caecum-colon junction) and distal colon (1 cm from the anus).
In all analyses described in the following, for evaluation of EEG changes during task execution, 30 EEG segments were collected for two conditions (end of pre-sleep practice and immediately after sleep, see Fig. 1C, fading-blue bars).
