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The main source of variability was the segment being analyzed, which accounted for more than 20% of the overall variability.
Lineage-specific divergence was estimated by maximum likelihood using PAML v3.14 [13] and was reported as a weighted average over each D. simulans line with greater than 20 codons in the segment being analyzed.
In effect, the values generated by this analysis represent the fraction of the total length of all axons detected that resides in the segment being analyzed, thus these normalized values are referred to form here on as quadrant fractional lengths.
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For viral genetic characterization, a partial fragment of G2-encoding region from the M segment was analyzed: genomic positions 2717 2943 (G2717 2943
Both diastolic and systolic cross-sectional areas of the studied segment were analyzed off-line by two observers unaware of clinical and hemodynamic findings.
At the end of the segmentation procedure each remaining segment was analyzed and labeled as amplified/deleted if the log2 ratio values within the segment passed two user-defined filters, one for the average and one for the p-value (calculated with a t-test).
Accordingly, from the location on the hair strand considered to be chronologically equivalent to the time point identified by the distal end of the toenail sample, a hair segment was analyzed that included 30 days growth in the direction of the distal end of the hair segment as well as a period of growth representing 60 days towards the proximal end of the hair segment.
Nucleotide and deduced amino acid sequences obtained for each segment were analyzed in the National Center for Biotechnology Information database (www.ncbi.nlm.nih.gov/Database/) to determine the percentage of identity with mammalian reoviruses.
The frequency content of each recording in each segment was analyzed by Fast Fourier Transform analysis (FFT) and the power spectrum was quantified by measuring MNF and MDF.
Sequences of each gene segment were analyzed with the Sequencher program (Gene Codes Corp., Inc., Ann Arbor, MI) and subsequently compared with other sequences by using E-CLUSTAL W and analyzed by using the DNAdist and Neighbor programs from PHYLIP software accessed through BioManager, Australian Genomic Information Service (ANGIS, University of Sydney).
An investigation was made by each actor into the nature of the context to identify what action was appropriate in each segment, and as part of this investigation, each segment was analyzed to determine the changing nature of crisis.
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