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Visualization of the c-Met or HGF on the sections was done using an immunoperoxidase system (Vector labs, Burlingame, CA) as per manufacturer's instructions.
Blocking of the sections was done in 5% TopBlock, incubation with primary (POM1: 1∶10'000, 4A6: 1∶1000) and secondary antibodies (Dako D0486, AP goat anti mouse, 1∶1000) were done in 1% TopBlock, respectively.
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"All I know is that the section is done," O'Halloran said.
The search for BMs presented in the previous sections was done on the consensus sequences of various Alu subfamilies.
The histomorphological evaluation of the liver sections was done by a pathologist unacquainted with the treatment groups.
The evaluation of the FISH sections was done simultaneously by two observers.
The rotational averaging of the calculated results has been performed following available literature, and the calculation of the cross sections was done using an approach that has recently been generalized by two of us based on work by Peticolas.
Immunohistochemical analysis of the primary tumor sections was done using an anti-CXCL-8 mouse monoclonal antibody to evaluate the expression of CXCL-8.
The analysis of all tissue sections was done without any prior knowledge of the clinical parameters by two authors (AC, GFZ) by means of light microscopy.
The evaluation of all IHC sections was done simultaneously by three pathologists, blinded as to the patients' clinical characteristics and survival data.
The evaluation of all IHC sections was done by experienced breast cancer pathologists, blinded to the patient clinical characteristics and survival data.
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