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Among the examples previously shown in the sections 'Time dependence', 'Polarization analysis of bursty-patches', and 'Chorus structures', only the one corresponding to the very long event on 19 February gave satisfying results for both stations (Figure 8b).
All the experimental conditions including buffers, fixative, thickness of the sections, time and temperature of the incubation, and dilution of the antibodies were kept constant for processing of the brains and pituitary.
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(For a history of this development, see the section Time-sharing from Project MAC to UNIX).
It has been pointed out to me that one of the section times available is Thursdays from 10 30 to 11 20, which is, of course, when the lecture meets.
(For a history of this development, see the section Time-sharing from Project MAC to UNIX). Time-sharing allows many people to interact with a computer at once, each getting a small portion of the CPU's time.
As we detail in the next section, time variation is important for the identification assumptions of our empirical model.
The resulting complex was visualized using 0.03% 3,3 diaminobenzidine (DAB) after washing the sections three times in PBS-T.
The reactions were stopped by washing the sections three times for 5 min in PBS.
Excess antibody was removed by washing the sections three times each for 5 min with PBS.
International Atomic Time (TAI; introduced in 1955) is covered in the section Atomic time.
We use these two time-courses as the replicates (removing the 1st section of the 13 section time-course) and use developmental time as the known time scale.
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