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The 14 amino acid avitag functions as defined biotinylation site that in combination with the biotin ligase BirA expressed from the second expression unit is quantitatively biotinylated in vivo in E. coli in the presence of 40 μM biotin.
In contrast, the second expression is designed to also work in the heteroscedastic case.
The second expression system utilised the high expression vector directly, Ig domains of the chosen immunoglobulin isotype were amplified from peripheral blood mononuclear cell (PBMC) RNA and ligated into the vector in frame with DNA encoding the antigen.
From the second expression, we obtain the equality 0=0.
For the second expression 4 × (3 × 2), you have how many groups of what?
where the second expression above is to meet the conditions that u i ∈ [0, 1].
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That was the first expression of a theory that would later become notorious.
Yet it is actually the first expression that comes to Fernandinho's mind when analysing … Barcelona.
110 would indicate the third expression or'd with the second.
"The British Museum was the first expression of the citizen's right to understand the world," Mr. MacGregor said.
But once he had spotted the first expression, he soon found three more examples in that same interview.
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