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Exact(17)
If the ratio of correctly aligned bases between any such alignment and the second alignment was >0.8 and the subject regions didn't overlap, then that part was considered to be ambiguously mapped.
The second alignment would increase the length of the road by 19.9 mile.
The second alignment method, MAFFT [27], was fully automated.
The second alignment is based on RDP Infernal which allows secondary structure alignment [42].
Hence, we prefer the second alignment as it creates the smallest visual distortion via maximum alignment.
Only alignments with ≥98% identity or only one mismatch, with the second alignment scoring worst than the first were accepted for analysis.
Similar(43)
BWA alignments were performed using the bwa aln command with options –n 1, −o 0, and –e 0 and bwa samse with option –n 4. When doing a second alignment, the customized reference was updated, if necessary, to contain one of the alleles at predicted heterozygous sites from the first alignment, sequences were aligned, and the alignments were filtered as before.
Finally, the tools based on Direct fragmentation do not directly exploit paired-end information; they fragment every read before the first alignment and find fusion candidates aligning those fragments to a genomic reference.
Common devices used in the first alignment turns are collaborative completion and repetition of the elements of the prior turn, but alliances can also be initiated through non-verbal devices such as head-shakes.
RIDOT eventually constructed the fourth alignment, with the freeway beginning north of the I-295 interchange and proceeding north to the border between Woonsocket and Cumberland.
* The third alignment was a spur in the Buffalo area that followed NY 33's pre-Kensington Expressway routing on Genesee Street.
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