Sentence examples for the sec step from inspiring English sources

"We cooperated with the SEC step by step in this investigation," says eBay spokesman Kevin Pursglove.

After the SEC step, Coomassie blue staining of ∼400 ng of total protein revealed a faint 14 kDa band (the approximate molecular weight of the αSyn monomer).

RsTSPO was also solublized and purified in Fos-Choline-12 (2 and 0.2%) using the same buffer conditions but without the SEC step.

A protein that was invariably present through the early stages of the purification was βSyn, which copurified with αSyn through the SEC step.

Cross-linking of the material following the SEC step (when little or no ammonium sulfate remains) revealed very small amounts of monomers (14 kDa) and the 60, 80, and 100 kDa multimers, in accord with our previous observations of cross-linked αSyn from intact normal neurons and from minced, post-mortem brain bits.

PCR was performed with a Bio-Rad iCycler and the following program: 35 cycles: step 1: 95° for 10 sec; step 2: 60° for 30 sec; step 3: 72° for 40 sec each cycle.

Using Matlab (MathWorks, Natick, MA), a sliding window Fourier transform was applied to the LFP signal using a 2 sec window with a 1 sec step.

Comparison of the analytical solution (3.5) and the numerical solution (3.6) of the fractional differential equation (3.1) for the parameters,, zero initial condition,  sec,, and is depicted in Figure 1. Figure 1 Comparison of analytical and numerical solutions of fractional-order viscoelastic models of cell (3. 3) for simulation time 5 sec, step, and in (3.6).

The following PCR temperature cycles were used (45 cycles were performed): Step 1 95°CC, 5 min; Step 2 95°CC, 10 sec; Step 3 55-60°CC (primer dependent), 10 sec; Step 4 72°CC, 10 sec; Step 5 4°CC, hold.

Cycling conditions for intronless GLTP were: step1, 94°C for 3 min; step 2, 94°C for 30 sec; step 3, 50°C for 30 sec; step 4, 72°C for 30 sec; step 5, repeat 36 times; and step 6, extend at 72°C for 10 min. For GLTP, cycling conditions were the same except: step 2, 90°C for 30 sec and step 3, 55°C for 30 sec.

PCR reactions were either run on a 1605 Rapid Cycler (Idaho Technology, Idaho falls, ID) with a denaturation step of 1 min at 94°C followed by 35-40 cycles of 10 sec at 94°C, 10 sec at 5°C under the Tm of the oligonucleotide with the lowest Tm and 30 sec - 1 min at 72°C or on a BioRad C1000 thermal cycler using the same parameters except that 30 sec steps were used rather than 10 sec's.