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The screening sample that was used for gross validation of methods consisted of three individuals with previously verified large-scale aberrations.
We used published conventions for numbering days in each subject for ease of comparison to prior relevant publications: In WEAU and SUMA, day 0 (d0) marks the onset of symptoms; in CH40, d0 denotes the screening sample (symptom onset was self-reported to be 2 days earlier; Table S1).
The mean age of the screening sample was 12.5 years (SD ± 1.9).
One patient could not be assessed for this parameter as only the screening sample was available.
The observed distribution of prognostic factors for the 2441 cancer cases in the screening sample is shown in Table 2.
We attribute to type I error due to the small sample size used in the Screening Sample Set.
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Low conversions result from the low surface area of the screening samples, however, could be overcome by advanced synthesis protocols.
The screening samples were quantified by LC-DAD close to the isobestic point of 240 nm that should give fairly accurate results for the conversions.
We acknowledge The Norwegian Colorectal Cancer Prevention study (The NORCCAP study) for access to the screening samples.
Of the screening samples, 84% were later tested for the presence of GAD65, ICA512, and IAA measured by the micro method (mIAA).
Analysis of obtained chromatograms showed no interfering peaks even though the screening samples were approx. 33 times more concentrated than those prepared for confirmatory analysis.
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