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The samples were stained with calcofluor white.
The samples were stained with toluidine blue for 30 s, and washed twice with sterile water.
The samples were stained by the method of EnVision (enhance labeled polymer system).
The samples were stained with OsO4 to improve the image contrast.
The samples were stained with hematoxylin and eosin (HE), safranin-O, and toluidine blue.
The samples were stained and analyzed after gel electrophoresis for 90 min at 110 V.
After washing the cells, the samples were stained with Hoechst (10 μg/mL, 300 μL).
Finally, after removal of paraffine, the samples were stained with H&E (hematoxylin-eosin) and microscopically analyzed.
The samples were stained with Mayer's Haematoxylin-eosin and Alcian blue- van Gieson solution (Baranto et al. 2005a; Baranto et al. 2005b).
After sectioning, the samples were stained with uranyl acetate and lead and observed with a JEOL JEM 100 S electron microscope at 80 kV (Miyoshi et al.[2003]).[2003]
The samples were stained with uranyl acetate and Reynolds' lead citrate, and observed under a transmission electron microscope H-600IVV, httpchi, http://www.hitachi.com/).hitachi.com/
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