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We used the Affymetrix GCOS (Santa Clara, CA, USA) software to convert the CEL files into absolute statistical signals and the samples were scaled to the average signal of 100.
To allow comparison of the scores within a process and prevent quantitative comparisons between processes, the samples were scaled such that the PLS-DA scores from the subjects treated with the dietary mix group have mean 0 (zero) and the scores from the control group have mean 1 (one) for all three axes.
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The pixel size on the sample is scaled by the optics too; by the ×15 Cassegrain objective/condenser, the effective pixel size over the sample (i.e. optical sampling) is 2.6 μm.
The input samples were scaled into the [0,1] range using the global minimum and maximum of the data.
In the unsupervised two-dimensional cluster analysis, the 123 miRNA expression profiles for 102 leukaemia samples were scaled to the median of the normal bone marrow controls.
To identify hormone refractory dependent expression changes MET_HR samples were scaled to the median across the non-refractory samples MET_HN.
Based on the characterization results, PETI-330/MWCNT samples were scaled up to ∼300 g and used to fabricate moldings by injecting the mixtures at 260 280 °C into a stainless steel tool followed by curing for 1 h at 371 °C.
The normalization procedure amplified every stimulus item such that the digitalized sample with the maximum amplitude was set at the maximum positive or negative value of the converter range, and all other samples were scaled proportionally.
Likewise all metastatic samples were scaled to the median across all primary tumour samples.
Samples were scaled to a positive (Gpr20 ) control region.
In the case of dataset GSE3325 each signal intensity from the primary tumour samples was scaled to the corresponding median gene signal intensity across the benign tumour samples.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com