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All the samples were quantified by HPLC-DAD and analyzed by CG-MS.
Carotenoid and chlorophyll contents in the samples were quantified by high-pressure liquid chromatography (HPLC) and a modified carotenoid/chlorophyll-specific extraction protocol45.
The samples were quantified by comparison to known standards purchased from Sigma Aldrich.
The nitrite levels in all the samples were quantified according to the standard graph of sodium nitrite.
After purification, the samples were quantified using a Nano Drop spectrophotometer (Nano Drop Technologies, Wilmington, DE, USA).
The elemental (Ca, Cu, Fe, K, Mg, Na and P) compositions of the samples were quantified by inductively coupled plasma-optical emission spectroscopy (ICP-OES, Perkin Elmer) [29].
The samples were quantified by HPLC (LC 1100, Agilent Technologies, Santa Clara, CA, USA) using a reverse-phase C-18 column at 25 °C.
The compounds in the samples were quantified by comparing density of the peaks and their areas (expressed as intensity per mm2) from the samples with those from standard solutions of rutin, qurecetin, ellagic acid, and myricetin on the same plate.
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The radioactivity of the samples was quantified using a gamma counter (Packard Instruments).
DTX in the samples was quantified by HPLC using the above method.
Amount of the virus in the samples was quantified based on this standard curve.
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