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The samples were probed using specific antibodies for PXN (Invitrogen, AHO0492), Phospho-PXN (Abcam, 4832), FAK (Millipore, 05 537), Phospho-FAK (Invitrogen, 44624G), Bcl-2 (Epitomics, 1017 1) COX-IV (Cell Signaling, 4844), DRP-1 (Novus Biologicals, NB110 55288), and MFN-2 (Epitomics, 3272 1).
The transmission of the sample was probed using the broadband (850 1050 nm) output of a home-built noncollinear optical parametric amplifier.
Following previously described protocols, the transcriptional profile of samples were probed using Affymetrix HG-U 133 Plus 2.0 chips representing over 47,000 transcripts and variants using more than 54,000 probesets.
Parallel samples were probed using an anti-actin antibody (Chemicon, Billerica, MA, USA) to confirm the equal loading of lysates between lanes.
Genomic DNA samples from somatic cells (blood cells) were probed using an Illumina HumanHap 550K genotyping chip.
Samples were probed by using either monoclonal antibody (mAb) SHA31 or mAb P4, and the signal was detected by using a Fluor-S multiImager (BioRad).
Sheet resistances (Rs) of the samples were measured using a four-probe resistivity test system.
The samples were normalized using Lowess and probes with missing values in 50% or more samples were removed from the analysis in Avadis V4.3 (Strand Genomics, Bangalore, India).
The samples were measured using a commercial dielectric probe (Agilent 85070D) and a network analyzer (E8361A).
Force measurements of the samples were conducted using a SiO2 colloidal probe (tip velocity 1000 nm/s, compressive force 20 nN).
After washed with probe-free medium, the samples were viewed using fluorescence microscopy (Olympus, IX71).
