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Briefly, the samples were performed using an Agilent 1200 high-performance liquid chromatography (HPLC) system combined with the AB SCIEX API-4000 quadrupole mass spectrometer as a detector.
Micromagnetic simulations of the samples were performed using the freely available OOMMF software (http://math.nist.gov/oommf/).nist.gov/oommf/
Subsequently, a biopsy and immunohistochemical staining of the samples were performed using a p65 or S100 antibody.
SDS-PAGE of the samples were performed using standard protocols for conformation of protein binding efficiency [19].
Preliminary observations on the samples were performed using an Olympus SZ-40 stereomicroscope (10 and 20× objectives) equipped with an Olympus DP10 digital camera.
In vitro cell culture studies of the samples were performed using human osteosarcoma U2-OS celinesnes and found a significant improvement in cell viability and proliferation.
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The characterization of the samples was performed using nuclear reaction analysis and Rutherford backscattering spectroscopy.
A complete morphological characterization of the samples was performed using XRD, TEM, SEM, N2 adsorption and surface hydrophilicity measurements.
Analysis of the fracture surface of the samples was performed using a scanning electron microscope EM 535.
The electrochemical test of the samples was performed using potentiodynamic method with a potentiostat coupled to PC (IVIUM-Compact-state, 20250).
Thermogravimetric (TG /differential thermogravimetric (DTG) analysis of the samples was performed using a MOM Q-1500 derivatograPaulikulik and Erdey, Hungary) in a temperature interval of 20 1000 °С.
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