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After overnight dialysis against 100 mM sodium phosphate buffer, pH7.0, the samples were concentrated using Amicon Ultra-15 100,000 MWCO spin concentrators as described by the manufacturer.
The samples were concentrated to approximately 10µL under vacuum at room temperature, and further concentrated and de-salted in C-18 reverse phase Zip Tip columns (Millipore).
Prior to iTRAQ labeling, the samples were concentrated and desalted using 10 kDa molecular weight cutoff spin concentrators (Millipore).
Because the samples were concentrated during digestion, the result obtained was calculated to get the actual concentration.
Because the samples were concentrated during digestion, the result obtained was calculated to get the actual concentration value.
Post extraction, the samples were concentrated on rotavapor and analyzed by HPLC.
Similar(26)
In alluvium formation, samples are clustered in the fields of 2, 3 and 4, and the majority of the samples are concentrated in the Na Cl type (Fig. 3), indicating the saline nature in the groundwater (Prasanna et al. 2010).
In POM, samples are clustered in the fields of 1, 2, 3, 4 and 5, and the majority of the samples are concentrated in the Ca Mg Cl type and mixed Ca Cl type.
In Tertiary and Lower Cuddalore Formation, the dominant fields are 1, 2 and 4, and the majority of the samples are concentrated in Ca HCO3 facies indicating the dominance of freshwater recharge into the aquifers.
The sample was concentrated to a final concentration of approximately 0.1 mM.
The sample was concentrated using a Vivaspin 20 concentrator with a 10-kDa-molecular-size cutoff (Vivascience) and loaded onto a Sephadex S200 gel filtration column (GE Healthcare).
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