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The samples were amplified using a T100™ Thermal Cycler (BioRad Laboratories, Hercules, CA, USA) with the following conditions: initial denaturation at 94 °C for 3 min; 5 cycles of 94 °C for 30 s, 45 °C for 20 s, and 65 °C for 30 s; and 20 cycles of 90 °C for 30 s, 55 °C for 20 s, and 72 °C for 30 s, followed by a final elongation at 72 °C for 5 min.

The samples were amplified using an ABI 7000 Real Time PCR System (Applied Biosystems, Darmstdt, Germany).

Because of the low yield of total RNA the samples were amplified using the RiboAmp™ RNA Amplification Kit 0201 (Arcturus).

The samples were amplified using a Bio-Rad MyCycler™ thermal cycler (Bio-Rad Laboratories).

The samples were amplified using the buffer kit plus 1.25 units Taq polymerase (Invitrogen, Carlsbad, CA, USA).

The samples were amplified using FastStart DNA Master SYBR Green 1 (Roche diagnostics) according to the protocol of the manufacturer.

After adding the Mo Bio IRS solution samples were placed at 4°C for 5 min. Genomic DNA from these samples were amplified using the Qiagen REPLI-g® Midi kit.

The loss of both alleles was confirmed when the sample was amplified using PowerPlex® 1.2 primers and resulted in a 9, 10 genotype at this locus.

The mtDNA samples were amplified using the primers in 25 µl PCR reactions containing 50 µM dNTPs, 0.2 µM of each primer, 1 µl template, 2.5 µl Taq polymerase buffer, and 1 U Taq polymerase (TIANGEN, China).

The cDNA samples were amplified using the Applied Biosystems GeneAmp RNA PCR Core Kit Foster Cityy, CA).

The control samples were amplified using the same conditions as those described above.