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It is evident from Figure1b that no diffraction peaks are observed for the samples (buffer layer thickness 8.9 nm) annealed at 600°C, whereas it shows well-defined peaks for films annealed at 700°C.
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After heating, the sample buffer containing the immunoprecipitated and associated proteins was transferred to a separate tube for Western blot.
The interference could be blocked by the addition of rat immunoglobulin to the sample buffer.
The concentrated SFV1 virions were purified in 10 25% sucrose gradient (in the sample buffer) by rate zonal centrifugation (Beckman SW32 Ti rotor, 82,667 × g, 20 min, 15 °C), and the virus light-scattering zone was collected and further purified in 0.45 g ml−1 CsCl (in the sample buffer) by isopycnic gradient centrifugation (Beckman SW60 Ti rotor, 215,000 × g, 17 h, 15 °C).
If you have any doubt about the acceptability of the sample size, remove the sample buffer from the AHDC sample tubes and submit the sample in the dry tube.
The use of surfactants and solvents in the sample buffer did not result in recognition of the plasticizers.
The sample was diluted (1 1) with the sample buffer.
0.01 % Bromo Phenol Blue (BPB) was added to the sample buffer to monitor migration time [18].
After being homogenized within the sample buffer they were either stored in liquid nitrogen or lyophilized.
Samples (25 μg) were applied to the gels both with and without β-mercaptoethanol in the sample buffer.
Briefly, sera diluted 1 101 in the sample buffer were applied to the wells and incubated for 30 min.
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