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In our case, fast measurements were not required and therefore not performed (the sample was translated by a manual stage).
Typically, 20 complete rotations were performed while the sample was translated over a distance of ∼500 μm.
The sample was translated at a constant velocity of 1.2 μm/s in order to acquire a series of z-sections at intervals of approximately 0.65 μm.
The axial response was measured from the change in signal at the pixel in the transverse centre of the microsphere as the sample was translated axially in 0.2 μm steps.
In order to acquire spatially and spectrally resolved intensity distributions of a specific area on a sample, the sample was translated step-wise in the focal plane of the microscope with a minimum step size of 1μm using an automated, remote-controlled stage (Prior ES110).
In order to build up an image from a series of discrete measurements, the sample was translated using a closed-loop piezoelectric stage that can move independently along three orthogonal axes (LP100, Mad City Labs); x, y are in the transverse plane and z is along the optical or axial axis.
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The experimental setup of Figure 2 used in this experiment while the sample is translated along the z direction and the transmitted intensity is measured through a pinhole in the far field as a function of the sample position z, measured with respect to the focal plane.
The sample is translated through the full range in 100 nm intervals for calibration purposes.
The sample is translated laterally at a rate of 0.6 mm/s (~55 pulses per 33 µm spot).
Before and after the exposing, the Fe/Si(111 -7 111 -7H5OH sample was translated into the composition test chamber, respectively, where the sample was in situ tested by the GammadataScienta SES-100 X-ray photoelectron spectroscopy (XPS) system (Pleasanton, CA, USA).
After the deposition of Fe atoms, the Fe/Si(111 -7 111 -7H5OH sample was translated into the main chamber for STM observation.
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