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The concentration of ROS in the sample was quantified by its ability to inhibit the binding of the anti-ROS antibody to the immobilized ROS-BSA and subsequently the color intensity in the assay wells.
High molecular weight proteins, which would otherwise interfere with the NO assay were removed from the lysates with Ultrafree Biomax 10 kDa columns (Millipore) by centrifugation at 11,000 rpm for 15 min. Total NO in the sample was quantified using the Endogen Total Nitric Oxide Assay Kit (Pierce) according to the manufacturer's protocol.
The sample was quantified using picogreen fluorescence (Molecular Probes Inc., Invitrogen).
The optical density of the sample was quantified at 517 nm.
The sample was quantified by the Lowry method and subjected to SDS PAGE and detected with Coomassie Brilliant Blue.
The number of moles of each residue in the sample was quantified by linear interpolation from the calibration equation.
Similar(51)
The radioactivity of the samples was quantified using a gamma counter (Packard Instruments).
DTX in the samples was quantified by HPLC using the above method.
Amount of the virus in the samples was quantified based on this standard curve.
The DNA in the samples was quantified using an ND-1000 Spectrophotometer (NanoDrop Technologies Inc., USA).
The total protein content of the samples was quantified using a 2-D Quant kit.
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