The sample was injected at an injection pressure of 5.0 kPa for 3 sec (approximately 3 nL).
The sample was injected into the capillary by pressure injection for 3 s at 0.5 psi, and the separation of samples was driven with an applied voltage of 30 kV at 25 °C.
The sample was injected into a small volume sample injection vessel for GC-MS analysis, and the conditions were as follows.
Filtered solutions were degassed before the injection; then, 100 μL of the sample was injected for HPLC ICP MS measurements.
As the sample was injected into the GC, the sample first underwent gasification in the injection port liner, and then entered the capillary column with a split ratio of 90:1.
After protein precipitation, the sample was injected into the LC MS/MS system for direct measurement.
The TTR variant was incubated in 20 mM PBS buffer (pH 7.4) and 300 μL of the sample was injected to the column.
The TTR samples were incubated in 20 mM sodium acetate buffer (pH 4.4) and 300 μL of the sample was injected to the column after the pH was adjusted to 7. T and D denote tetramer and dimer, respectively.
In all experiments, 1 μL of the sample was injected (splitless), using He as the carrier gas onto a CycloSil-B column (Agilent, 30 m length, 0.25 mm inner diameter (i.d)., 0.25 μm film thickness, cat.
The sample was first injected on the first column of length 30 mm where less retained analytes were separated and then the sample was injected on the second column of length 10 mm where more retained analytes were separated.
The sample was injected at 300 °C.
