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The sample was acquired on the FACSCalibur and gated on the CD45 bright + lymphocytes.
The entire depth of the sample was acquired as 3D image stacks at approximately 20 µm thickness for each optical section.
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19F and 11B NMR spectra of the sample were acquired at each stage of the process.
Both temperature and magnetization data of the sample were acquired through a data acquisition card PCI-62511, NAustinl InsTXUSAnts, AUSAin, TX, USA).
24 scans of the sample were acquired.
Eventually, a mass chromatogram of the corresponding authentic standard and the sample were acquired and the peak submitted to fragmentation in a MS/MS experiment.
These artifacts can be avoided by placing the zero pathlength difference position outside of the sample, which results in two non-overlapping mirror images of the sample being acquired in the positive and negative frequencies.
The fluorescent signal from the samples was acquired at the end of the elongation step.
This is due to different place and, mainly, depth where the samples were acquired.
Scanning electron microscopy (SEM) images of the samples were acquired on a Carl Zeiss EVO-50 Low Vacuum SEM.
The samples were acquired by compacting sheet of nano-TiO2/potassium bromide powder mixture (1 100 in mass) and then drying at 110°C for 5 min.
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