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The sample solution was deposited dropwise onto a KBr disc.
After about 5 h standing, the sample solution was completed.
A 100-μL portion of the sample solution was injected.
The filtrate was obtained and used as the sample solution.
The pH of the sample solution was about 6.0.
The sample solution on this region was dried, and then measured by TXRF.
Time of the RBiABE contact with the sample solution is precisely controlled.
These results indicated that the sample solution was basically stable at room temperature within 24 h.
Finally, 1 mL of the sample solution was used to measure the thiol content.
As seen, the absorbance signal increases with increasing picric acid concentration in the sample solution.
The absorbance of the sample solution at λ1, λ2, λ3 and λ4 was determined by spectrophotometer.
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