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This was located in close proximity to the sample chamber.
D.C. and P.B. designed and fabricated the myosin supports and the sample chamber.
To reduce instrumental background, all samples were kept in the sample chamber overnight before analysis.
All samples were kept in the sample chamber overnight before analysis.
The bolometer was placed in a vacuum chamber separated from the sample chamber.
After 5 mins of adsorption the sample chamber was sealed and the quasi-static cycling was initiated.
The sample chamber can also be heated up to 900 °C by a radiative furnace.
Vacuum of the sample chamber was kept 10-4 Pa during the measurement.
The unit allows gas pressures in the order of 10 mbar in the sample chamber.
Approximately 2 μl of surfactant, 10 mg ml−1, was injected into the sample chamber and allowed to float by buoyance to the agarose ceiling.
This is because a small cylindrical furnace inside the sample chamber is used to control the sample background temperature.
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