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After three vigorous washings in the same washing buffer for 15 min, each membrane was incubated with a goat anti-mouse conjugated with horseradish peroxidase conjugate (1: 1000 dilution, Catalog No. A0216, Beyotime, China) at room temperature for 2 h.
The duvets, the pillow cases and the sheets are washed and ironed by the club, using the same washing powder and the same methods.
His father, a textile engineer, has maintained the same washing machine for 50 years, and his mother uses an etching press with a design that was popular in Rembrandt's time.
Then, 10 μL of phosphate buffer saline (PBS) solution (0.1 M, pH 7.2) containing 2% BSA (w/v) was dropped onto the electrode to block the area without the MAb attachment and the same washing steps were conducted after 30 min of incubation at room temperature.
After the same washing step, both the enzyme-embedded ZIF-8 (enzyme/ZIF-8) and enzyme-adsorbed ZIF-8 (enzyme@ZIF-8) samples were digested by acetic acid, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, Additional file 1) performed on an analytic polyacrylamide (12%) gel.
This bottle does the same, washing over your palate with sharp herbs, cutting acidity and a tease of fruit you can't quite have.
Similar(35)
"Kidnapped," "The Nine," "Smith" and still others have adopted the same washed-out palette, pewter shadows and amber light.
"Seventy percent of the time, it's the glass -- maybe there's still detergent on it, or they put it in the same wash as the haddock plates.
The slow-dance blueprint "Brown Eyes," co-written by Walter Afanasieff, who has applied the same wash of emotion to songs for Celine Dion and Barbra Streisand, could become the group's most ordinary hit.
A week later, the same washed out, eery photograph of Demi (or, reader, the person who we thought was Demi – so "Demi") was posted again to Tumblr, this time by user cstcrpt.
On the floor and other walls are my fellow subjects, all rendered in the same washed-out oil colours, minimalist detail to hair and clothing bringing out their vulnerability through their eyes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com