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The same transferred membrane was rinsed and reprobed using mouse anti-human β-actin to test the loading of total protein.
As control, on the same transferred membrane, β-actin was detected using mouse anti-β-actin (diluted in 1 1,000) (product code ab6276; Abcam, Cambridge, UK) and ECL sheep anti-mouse-IgG horseradish peroxidase-linked species-specific whole antibody (diluted in 1 2,000) (product code NA931; GE Healthcare) and all other similar steps as above.
The four strains of C. trachomatis (J 6276tet1, RC Fs 342, RC Fs 852, and RC Js 122) were found to possess the same transferred rRNA operon and shared the same genomic organization in the region of the transferred rRNA operon (supplementary fig. S4, Supplementary Material online).
As a control, on the same transferred membrane, beta-actin was detected using mouse anti-beta-actin primary antibody at 1 : 1000 dilution and ECL sheep anti-mouse-IgG HRP-linked species-specific whole antibody, diluted 1 : 5000 (Amersham Biosciences, Piscataway, NJ) and subjected to all other similar steps as above.
Similar(56)
The Scottish Nationalists proposed the same transfer last week.
The passive control (PC; n = 64) group did not engage in any training but completed the same transfer tasks.
Dias has improved steadily since joining in February and provides a composed presence alongside the slightly more erratic Biava, who arrived in the same transfer window.
"GMA and Piveteau were therefore signatories to two mandates of two different clubs for the same transfer, and that contravenes [regulations]," said the court verdict.
There's no indication in the Mail story that Willis wants the same transfer rights for any movies he's downloaded and stored in his iTunes archive.
The inference seems to be that double taxation-by two states on the same transfer-should be sustained in order to prevent escape from liability in exceptional cases.
To segregate two or more items is to keep them in separate accounts, and to avoid combining them in the same transfer between two accounts.
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CEO of Professional Science Editing for Scientists @ prosciediting.com