Exact(5)
Negative controls included substitution of the primary antibody with an unspecific antibody of the same subclass, as well as staining of cell lines previously known to be negative for S100A4, E-cadherin and the catenins.
The receptor c-KIT is a tyrosine kinase receptor, belonging to the same subclass as platelet derived growth factor receptor.
T. gondii, the only member of the genus Toxoplasma, belongs to the same subclass as the Eimeriidae, the Coccidia.
In controls, the primary antibodies were omitted or replaced with an irrelevant antibody of the same subclass as the specific mAb.
The α2 form is in the same subclass as ProRS, SerRS and ThrRS (subclass IIa), while the GlyRS heterotetramer groups with PheRS (subclass IIc), another α2β2 heterotetramer enzyme [ 36].
Similar(55)
Negative control included substitution of the monoclonal antibody with mouse myceloma protein of the same subclass and concentration as the monoclonal antibody.
Negative controls were performed by substitution of the primary antibody with immunoglobulins of the same subclass and concentration as the monoclonal antibody.
Mouse myeloma protein of the same subclass and concentration as the primary mouse anti-ALDH1 antibody was used for negative control.
Sections from normal skin with known expression of BRMS1 was used as positive control, whereas negative controls included substitution of monoclonal antibody with mouse myeloma protein of the same subclass and concentration as the monoclonal antibody.
All series included replacement of polyclonal primary antiserum with normal rabbit serum diluted 1 : 2000, whereas negative controls for the monoclonal antibody were performed using mouse myeloma protein of the same subclass and concentration as the monoclonal antibody.
Negative controls included substitution of the monoclonal antibody with mouse myeloma protein of the same subclass and concentration as the monoclonal antibody and substitution of the polyclonal primary antibody with normal rabbit IgG of the same concentration as the polyclonal antibody.
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