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Also, to compare the predictive power of GBLUP and ABLUP on the validation sets, the correlation and scatter plots between GEBV and EBV2 values for the same replicates were produced.

The analytical precision of the set-up was assessed by reproducibility achieved for repeated measurements of the same replicate and by the reproducibility achieved for repeated preparations and measurements of the same urine sample [40].

The analytical precision of the set-up was assessed by (a) reproducibility achieved for repeated measurement of the same replicate and (b) by the reproducibility achieved for repeated preparation and measurement of the same urine sample.

Observations were repeated with the same biological replicate for each tissue.

It is not necessary to use the same number of replicates for all sample types.

When possible, we used multiple individuals from the same colony as replicates for genome size estimates.

The same three replicates used for chemical analysis were also used for sensory analysis.

Bootstrap proportions were calculated based on 500 bootstrap replicates, using the same settings as for each replicate in the heuristic search, but with only 10 random stepwise addition sequences per replicate.

Total RNA was extracted from different samples obtained from the same three replicates used for microarray study using TRISure (Bioline, London, UK).

Water content of the same biological replicates used for CW analysis was determinated to show that the water content of the needles in all the experimental plot was not changed.

By rescaling the TBV in this way, their mean and variance were the same for each replicate and for the different numbers of QTL, which indicates that when the number of QTL was higher, each QTL explained a smaller part of the variance.

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