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In the same process as the Empirical Threshold, LOD scores are calculated for each resampled data set for each marker.
Follow the same process as listed above.
The same process was performed in the validation group.
Use the same process for the barrel.
Follow the same process for conceptual problems.
For validation, the same process was performed in the derivation group.
Then candidate proteins were selected for data validation based on the RNA-seq and qPCR analysis, and the protein levels were presented as fold change (Supplementary Data File).
Repeat the same processes for other loops.
Using the same data as for CC validation, the RNApred (Kumar et al., 2011) achieved accuracy of 82.5% for the positive set and 52.8% for the negative validation (Table 1; Supplementary Material).
The analytical process followed the same specifications as for simulated data sets.
A detailed overview of the data collection and calculation process, as well as the data validation process through several bunkering companies, is provided in Aronietis et al. (2015).
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