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A schematic of the structure of the RPDA is depicted in Figure 1a, at the same orientation for the measured patterns.
The directional loxP sites must be in the same orientation for excision of the intervening sequence to occur after recombination.
In cases where the primer is found in the reverse complement orientation, it is a good idea to reverse complement the sequence so that all reads are in the same orientation for the remaining analysis steps.
Interestingly, the gametic selections have the same orientation for male and female Clementine in the genomic regions where sweet orange segregations are also skewed (LG1, end of LG5, and LG9).
Large-scale chromosomal deletions and inversions can also be generated by placing loxP sites, either in the same orientation for deletions or the reverse orientation for inversions, further apart on the chromosome [154,155], while chromosomal translocations can be created by placing a loxP site at each breakpoint [156] on non-homologous chromosomes.
Since the relative orientation of the two transcriptional units was irrelevant to the expression and the biotinylation levels of the BAP-tagged protein (not shown), we arbitrarily chose the vector with the two cassettes in the same orientation for all the different molecules tested (listed in Fig. 1).
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3) Also, please plot the actual axes of orientation used to align the barrels on this summary image, and please make sure all maps are presented in this same orientation for consistency.
The stop codon of the Cre gene and the start codon of the EGFP gene were modified to contain loxP sites in the same orientation, allowing for the generation of a Cre-EGFP fusion product for colour detection after Cre-mediated excision of the intervening sequence.
Preoperative mean b = 0 images and distortion matched HR-EPI images were co-registered using SPM5 in order to ensure that they were in the same orientation (Wellcome Trust Centre for Imaging Neuroscience, UCL; http://www.fil.ion.ucl.ac.uk/spm).ac.uk/spm
In this orientation, neither the group at C3 (which has now exactly the same orientation as for nat-PS) nor the C18/C19 methyl groups (which now project to the same side) can hinder the binding (or the access) of ent-PS.
Diffusion MRI data was acquired in 31 axial 500-μm slices, with the same orientation as for the previous T2*- and T2-weighted scans, using a 4-shot DTEPI sequence [repetition time, 7750 ms; echo time, 20 ms; time between the application of diffusion gradient pulses, 14 ms; diffusion gradient duration, 7 ms].
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