For caspase-3 assay, muscle proteins were extracted with cell lysis buffer from the same mice as described above.
Last, our data might have been more valuablehad we been able to take sequential serum samples from the same mice as the disease progress.
Plasma from vaccinated mice with the highest titer to Aβ1-42 and PHF, as well as the pre-immune (T0) plasma from the same mice (as a control), was used for immunostaining of human tissue.
Muscle strength of the forelimbs of mice was measured by a Grip Strength Test using a Grip Strength Meter apparatus (TSE Systems Gmbh, Hamburg, Germany) for the same mice as in the Rotarod test.
shows weight loss of the same mice as shown in Figure 4(a).
Kirrel3 knockout mice used in this study are the same mice as described previously in Prince et al. 2013.
These are the same mice as those shown in (A, middle panel) and (B, middle panel), respectively.
These are the same mice as those shown in Figure 3A (middle panel), 3B (middle panel), and 3G.
In largely the same mice as used for CSF measurements, we then analyzed the amount of total brain Aβ by immunoassay and assessed the onset of Aβ deposition by Aβ immunohistochemistry (see Materials and Methods for details).
Because we [ 14] used the same mouse models as Silva et al. [ 15], the data quantifying mature crosslinks can be compared between the mandible and the femur.
