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The same localisation patterns were found upon expression of TP03_0882 containing a C-terminal V5 epitope tag, indicating that the nuclear accumulation is not an artefact caused by the eGFP fusion portion of the polypeptide.
Moreover, they showed the same localisation for TbKIN13-1 and TbKIN13-3 (nucleandand cytoplasmic, respectively) in cells containing either tagged or untagged protein (Fig. 3), demonstrating that the localisation of the GFP-tagged proteins was representative of that for the wild-type protein.
Promastigotes expressing LdARL-1-GFP showed the same localisation (Fig 3B, 4A); the green GFP signal was adjacent to the red one of mRed-LdLpg-2 signal, a Golgi cisternae marker [40] (Fig 3B) and co-localised with LdARF-1-mRed [10] (Fig 3C).
However, a recurrent episode was not confirmed as ventilation/perfusion scintigraphy revealed a perfusion defect on the same localisation as the previous pulmonary embolism.
Vice versa, the DSS rate was markedly increased to 74% for grade 2 cancer patients with low YB-1 protein expression at the same localisation.
Multiple RSR sequences mapped at more than one location and multiple RSR sequences mapped at the same localisation and thus corresponded to the same gene.
Similar(46)
Tagging the GFP to either the N or C terminal of PBGD exhibited the same nuclear localisation pattern, although the specific localising sequence is not yet defined.
These data show that levels of the C-terminally GFP-tagged kinesins are very close to untagged levels and that both tagged and untagged proteins have the same cellular localisation.
Although DLO pyrophosphatase activity has been identified in various microsome preparations [13], [19] we wanted to demonstrate that Man7GlcNAc2-P generation and ER-mediated polypeptide N-glycosylation [41] occur at the same subcellular localisation in permeabilised cells.
Furthermore, newborn rat brain cells grown for 20 h as a primary culture showed the same nuclear localisation of PBGD.
In addition, even patients with the same anatomical localisation and stage may experience different outcome and treatment morbidity on the same treatment modality.
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