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The following steps and conditions were the same described above for denaturing western blotting.
For free cell culture assays with all the Streptomyces spp. strains together, the methodology used was the same described above (see Section 2.4).
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The same steps as described above were then followed.
The focus of the observations was the same as described above for the acute toxicity study.
The remainder of the parameters were the same as described above.
The final reaction condition was the same as described above for the radioactive assays.
The primer sequences, the reaction setup and the cycling conditions were the same as described above.
The apparatus was the same as described above.
The procedure is the same as described above.
The rest of the procedure for immunofluorescence was the same as described above.
Also, the entropy was calculated for the 60 normal samples using the same procedure described above.
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