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"Perhaps we can attenuate the Zika virus by using the same deletions of the same sites," Kalil says, noting that Butantan likely would partner with NIAID to develop its vaccine.
Recombinant viruses of strain Ingelvac® PRRS porcine reproductive and respiratory syndrome virus (PRRSV) modified live virus vaccine were produced with two individual small in-frame deletions in nonstructural protein 2 (nsp2; Δ23 and Δ87) and also the same deletions supplanted with foreign tags (Δ23-V5, Δ23-FLAG, Δ23-S, Δ87-FLAGΔ87-SLAG, Δ87-S).
To do this, we constructed a full length MHC-IIB fused to GFP on its N-terminus, with the same deletions as the Rod mutants (Figure 6A).
The same deletions were also observed in a fraction of RT-PCR products generated from MCF10CA1h cells, which is consistent with the partial deletion in DNA copy number suggested in the analysis shown in Figure 2A.
However, the same deletions were characterized by significant synergistic effects in the repression of SMs in a delta hdaA background.
Haplotype analysis of the kindreds sharing the same deletions failed to show evidence of a founder effect (Wijnen et al, 1998b).
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Our finding identified the same deletion in CbmNav1.6b of mouse cochlea (Fig. 1E).
And voilà, the same deletion was present in 102 of the 104 black wolves from Yellowstone the team tested and in all nine black wolves from the Canadian Arctic, Barsh and colleagues report online today in Science.
Satisfied that they had fingered the genetic cause of this evolutionary change, Bargmann's group wanted to see if the same deletion was involved in other cases of dauer loss under crowded lab conditions.
Of these, 274 had the same deletion called by PennCNV in one or the other parent.
Previous studies have reported a high correlation for IQ values in affected siblings with the same deletion mutation, but a poor correlation between unrelated affected individuals with the same deletion.
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