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Unattended operation of the instrument revealed exceptional overall performance: Reproducibility (1 5% deviation in uncorrected elution times), repeatability (<20% deviation in detected abundances for more abundant peptides from the same aliquot analyzed a few weeks apart), and robustness (high-throughput operation for 5 months without significant downtime).
Endogenous control samples were loaded on the same plate as the test samples, from the same aliquot of cDNA.
Firefly and Gaussia luciferase activities were measured in the same aliquot using an automatic luminometer equipped with an injector device (Victor III, Wallac and Tristar, Berthold).
This example of the integrity of RPM-Flu assay-generated target pathogen gene sequencing for detection and identification is reinforced by results for the other specific hemagglutinin, neuraminidase, and matrix gene sequences from the same RPM-Flu assay of the same aliquot of FluMist vaccine.
Viral RNA was extracted from virus culture supernatant (the same aliquot used for neutralization) as described by Boom et al, [42] followed by reverse transcription using Oligo dT primer (Promega Corporation, Madison, WI) and AMV reverse transcriptase (Access RT-PCR system; Promega Corporation, Madison, WI).
Second step of polymerase chain reactions were performed with the same aliquot of first-strand cDNA as a template.
Similar(48)
The same aliquots of MNPs in saline containing 50% mouse blood were added into crucibles on a magnetic field to collect MNPs and washed two times with saline.
The same aliquots of MNPs, 300 μl in saline, were respectively mixed with 200 μl of saline and 200 μl of saline containing 50% mouse blood.
The same aliquots of total RNA samples used in the microarray experiments were utilised for this analysis.
This result is in agreement with the SDS-PAGE analysis of the same aliquots, where no apparent differences were observed comparing the amount of soluble material before and after ultracentrifugation, after removing the fibrillar material.
Our data disclosed that the analysis of the same aliquots of the 82 selected lymph nodes initially characterised by HE led to 100% specificity by employing qRT-PCR and the CK19 biomarker, while in contrast, the same methodology utilising the tumor-specific marker CA9 failed to recapitulate a similar pattern of diagnostic accuracy.
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