Running was terminated when mice touched the electric shock grid at the back of the treadmill more than 10 times/min, and the total running distance was recorded.
After little more than a year at the club, McCarthy's contract, which still had 14 months left to run, was terminated for £1.5m, prompting West Ham vice-chairman Karren Brady to describe the player "as a big fat mistake" in her newspaper column.
The run was terminated at 6.723.300 generations, when the average standard deviation of split frequencies between the two runs was <0.01, and examination of the p-files in the program Tracer 1.4 (http://tree.bio.ed.ac.uk/software/tracer/) showed that the two runs had converged.
The cycling conditions for the TaqMan® assay were 94°C for 2 min followed by 40 cycles of 94°C for 15 s and 60°C for 1 min. The cycling conditions for the SYBR® Green assays were the same but the run was terminated by a melting curve analysis where the fluorescence was continually measured during a temperature increase from 60°C to 95°C.
The run was terminated when the dye front reached the lower end of the gel.
After 24 h, no further change could be detected in the scans, and the run was terminated.
Five ML search replicates were run for each dataset using random starting trees, and up to five million generations were employed for each run unless the scoring topology lnL did not improve by ≥ 0.01 for 20 000 generations, in which case the run was terminated prematurely and the next bootstrap replicate was begun.
The run was terminated only after the Bayesian MCMC searches had reached a stationary phase (plateau), indicating convergence of the chain onto the target distribution, and a consensus tree built using the last 1000 trees (burn-in = 49001 samples for main tree, 9001 samples for subset analyses).
For each run, only one pass through the list of URLs was made, the run being terminated when a few thousand successful measurements (samples) were completed (the lists of URLs from the caches contained hundreds of thousands of URLs and so were not exhausted).
The runs were terminated by switching off the power and the experiments were quenched rapidly (∼50 °C/s).
