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Given the robust changes in adult neurogenesis evident in both disorders, competing effects on brain structure are likely.
Given the apparent impermeability of BBB to GDF11 (Fig. 4h), the rapid absorption of GDF11 into the blood (Fig. 4a), and the robust changes we observed in the cerebral vasculature in response to systemic GDF11 treatment11 (Fig. 2), we hypothesized that endothelial cells that line the luminal surface of vessels are a target of GDF11.
The robust changes in cytokine expression and histopathology after rCARDS toxin treatment suggest that CARDS toxin has the potential to induce significant immune pathology in the lung.
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Further, the robust change in non-synaptic proteins suggests that AIE may prime this signaling pathway for future ethanol exposures in adulthood.
Lifetimes as determined by TCSPC were generally in reasonable agreement with the frequency-domain data and confirmed the robust change in FRET following elevation of cAMP levels (Fig. 1F).
To find the genes with the most robust changes in expression, the data was plotted as a "Volcano Plot" (Supplementary Figure S2B), which allows statistical significance to be measured along with the extent of fold change in expression.
This adds a randomization to the tissue group comparisons which should act as a filter to identify the more robust changes due to the disease by decreasing noise due to chance differences in heritable gene expression levels between individuals.
In summary, the changes in gene expression we detected and validated represent the most robust changes, which are neither in the feeding regulation nor the stress response categories.
Steady-state fluorescence titrations of AtCaM6 with the W980 peptide in the presence of Ca2+ were measured because the AtCaM6 W980 peptide combination gave the most robust changes in fluorescence.
They all show the low potassium, high magnesium profile that we discuss as being the most robust changes.
But they collectively resisted some of the more robust changes that have been proposed.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com