Sentence examples for the revised sequence from inspiring English sources
We then realigned the reads against the revised sequence.
Similarly, seven gaps were present when L er-0 whole-genome reads were aligned to the revised sequence.
We wish to thank Vicente M. Cabrera for providing the revised sequences from several of his studies, and Alexei Drummond and Simon Ho for guidance on BSP analyses and helpful discussions, as well as Phillip Endicott.
Finally, the revised sequences were compared with all the DyP sequences (a total of 218) from the 114 basidiomycete (Agaricomycotina) genomes currently (7 December 2014) sequenced and annotated at JGI, which are available at the MycoCosm portal (http://genome.jgi-psf.org/programs/fungi).
The absence of misassembly signatures overall demonstrates that the L er-0 revised sequence is superior to the draft sequence.
To evaluate the accuracy of the L er-0 revised sequence, locus-specific reads were mapped to all three sequences (Col-0, L er-0 draft, and L er-0 revised) using the most stringent parameters (no gaps and no ambiguities were allowed).
In comparison to the L er-0 draft, the L er-0 revised sequence is a better reference (table 2).
From the stringent alignment of locus-specific reads to the L er-0 revised sequence, seven gaps were identified (six gaps corresponding to unamplified regions and one gap to the aforementioned unresolved region).
Sequence data were compared with the revised Cambridge sequence (GenBank Accession No. NC_012920.1) and sequences present in MITOMAP (http://mitomap.org/MITOMAP) and mtSNP (http://mtsnp.tmig.or.jp/mtsnp/index_e.shtml) using SeqScape software (Applied Biosystems).
The conventional sequence used as a basis of comparison for the determination of mtDNA haplotype is the revised Anderson sequence also known as the revised Cambridge Reference Sequence (rCRS) which is available at http://www.mitomap.org/.
The revised title sequence underscores this decline with a host of allusions to the programme's glory days - a three-eyed crow recalls Blinky the fish; the statue of Jebediah Springfield suffers another decapitation; Sideshow Bob yet again swipes in vain at his elusive nemesis Bart.
