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The cation exchange method showed greater sensitivity and tolerance to low pH samples than the reversed phase method.
The reversed phase chromatography utilizes a monolithic C-18 modified silica column, which produces fast and efficient analyses.
The reversed phase column was maintained at 40 °C in the column oven whereas the SCX column was kept at ambient temperature.
(a) o-DCB, 135°C, 10 min. (b) NaOH, DMF, 110°C, 5 min. Figure 3 Chromatogram from the reversed phase semi-preparative HPLC purification of [ 18 F]FE-DTBZ-d4.
Electrospray ionization in negative mode was used to ionize compounds separated by the reversed phase C18 column.
2 µg of total peptide at 0.1 µg/µL were loaded onto the reversed phase column using a two-mobile-phase solvent system consisting of 0.4% acetic acid in water (A) and 0.4% acetic acid in acetonitrile (B).
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In addition, superior performance for the reversed-phase separation of proteins requires that specific design properties for the bonded-phase should be incorporated.
These columns were used in order to determine their suitability for the reversed-phase separation of small molecules in isocratic nano-LC mode.
In parallel, the porous and hydrodynamic properties of the derived monolithic columns were thoroughly studied in isocratic nano-LC mode for the reversed-phase separation of a homologous series of small retained molecules.
The reversed-phase separation was performed as described previously[21].
Glucose responsive microgels were fabricated by the reversed-phase emulsion crosslinking method.
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