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We created a new riboregulator consisting of the reverse complement of a known riboregulator.
Surprisingly, HCV-IRES and the reverse complement of PARS1 (PARS1rc) were both found to display significant promoter activity as shown by 5′ RACE.
RD4-GFP-AS was the reverse complement of the previous.
The reverse complement of the input core motif is also scanned for.
As a reporter control we cloned the reverse complement of the miR-132 targets into the mCherry UTR (Fig. 1b).
Essentially the same results were obtained using probes for the reverse complement of siR1003 (data not shown).
We search for sites, both on the sequences provided, and on the reverse complement of those sequences.
Each microarray contains the reverse complement of 866 miRNAs and mature star sequences as published in the Sanger mirBase 12.0.
If no matches are detected at these positions, 'galign' generates the reverse complement of the input fragment, and repeats the above algorithm.
Lemma 2: If the reverse compliment is considered for finding MAWs, then the reverse complement of a MAW is also a MAW.
Furthermore, we designed divergent forward primers as the reverse complement of convergent reverse primers, and divergent reverse primers as the reverse complement of convergent forward primers.
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