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At the stage of implementation, they will play a key role in revising existing curricula, textbooks, or tests or designing new ones based on the results of alignment studies.
The average sequencing depth and coverage was calculated using the results of alignment.
A consensus sequence for the p20 and p10 domains was established based on the results of alignment.
Here, we describe the Fraxinus game, the results of alignment comparisons, the response the game received, and details of parameters fitted to replicate player dynamics.
In addition, considering that subsequence rearrangements are normal during the biological evolution process, if there are too many rearrangements in the sequences, the results of alignment based methods may be unreliable.
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Duplicate hits were manually removed before sequences were aligned using M-Coffee [ 110], combining the results of alignments using Muscle [ 111], Mafft [ 112], ProbCons [ 113], PCMA [ 114], and Fsa [ 115].
The results of alignments all perfectly matched with DNA sequences anticipated after sequencing PCR products (data not shown).
The results of alignments also revealed that Glu141, Glu237, Glu238, Glu292 and Glu591 were conserved amino acids among these GHF5 β-mannosidases.
The protein distance matrix method we have developed for phylogeny reconstruction utilizes all available sequence information and calculates protein distances from the results of alignments of protein sequences between species.
Such larger gaps could also be the result of alignment errors.
LCBs that contained a bias of single nucleotide polymorphism (SNP) density toward the edge of the alignment could have been the result of alignment artifacts.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com