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The rest of procedures for invasion assay were the same as in the migration assay.
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As control, adipocytes slides were not incubated with the primary antibodies, but with IgG isotype 1 and the rest of procedure remained unchanged.
RNase A (100 ug/ml final concentration) was added to the precleared extracts before immunoprecipitation and the rest of procedure was performed as described above.
While most statistics detect that admissions for hip fracture and appendectomy seem to have the lowest point estimates, the χ, Bohning and Dean tests suggest that pacemaker implant or lower extremity amputation, the two procedures with the lowest rates, appear to have lower point estimates than those obtained for the rest of the procedures.
We await the results of randomized controlled trials to further determine what role eye movements and other bilateral stimulation make to treatment outcome independent of the rest of E.M.D.R. procedures.
Then the rest of the procedures were followed as above.
For the rest of HO procedures, UTRAN is attached to IR1 whereas WLAN is attached to IR2.
In order to settle matters, a team was sent to Slatina by Zagreb University's Institute for Forensic Medicine to perform several autopsies, retrieve bodies and perform the rest of the procedures in Zagreb.
The rest of the procedures were performed as described [ 23].
The rest of the procedures were the same as described previously.
The rest of the procedures were similar to cryo-section hybridization protocol.
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