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To define the replicated time zero (t0) samples, we obtained two independent samples of 0.5 ml (i.e., 7.5 × 10 cells) from the pooled library.
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In this paper, described p value cutoffs are the averages of the two COSOPT-generated p values from each of the replicate time courses.
Concluding the SNP calling data on the technical replicates, we have a total of 1228 SNP positions (the sum of the number of SNP positions per sample across the replicates times the number of replicates for that sample) in all the technical replicates.
For any described s value cutoff, the average of the s values returned from the two replicate time courses is considered.
The screening was replicated three times over time.
A RSM was fit to data on the maximum biomass (K, estimated from each of the three replicate time series).
For this, we assume that each of the replicated time-courses is independently and identically distributed according to (3) (4) where and represent the gene expression vector which is measured by the l-th replicate and the corresponding state vector at time n, respectively.
The experiment was replicated several times and each time around 400 nuclei was screened.
The experiment was replicated three times with three replications each time while each treatment contained 21 plants.
After pre-treatment, 15 adult earthworms were introduced into the container, replicated six times.
A complete randomized block design with the hybrids replicated four times was adopted.
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