Exact(8)
The replicated samples produced only one discordant genotype among 107,412 genotypes indicating an assay replication success exceeding 99.99%.
In grassland soils, similar values for estimated bacterial richness were obtained for the three samples derived from fertilized mown pastures grazed by horse and cattle whereas the replicated samples from the other two management types showed strong variations in estimated bacterial richness (Figure 2 and Table S3).
All the replicated samples exhibited identical results.
A linear model was fitted to the expression values of each probe, modified to account for the replicated samples and assuming no interaction between the genes.
It also cannot be guarantee that the replicated samples in terms of biological, technical and analytical aspects reproduce the same results, even in a qualitative sense.
Also, none of the obtained data contain exactly the same values even in the replicated samples, mainly due to high biological variation among samples.
Allele calls from the replicated samples of amplified Akcent and the unamplified sample of Akcent were analyzed for concordance and the reproducibility was evaluated by calculating the coefficient of variation (CV, standard deviation as a percentage of the mean).
The range of fragments scored for each primer-enzyme combination is given in the Additional file 2. The replicated samples allowed the removing of non-reproducible bins and subsequently, the calculating of the error rate according to Bonin et al. [ 13] with RawGeno v 2.0.
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